Generator

Part:BBa_K106688

Designed by: Jacinto Chen   Group: iGEM08_UCSF   (2008-10-28)


500bp Distance Silencing

Cyc1P-GFP-Adh1t-500bp Spacer-8xLexA Operator.

For testing the distance of silencing. Contained in the yeast pRS306 integration vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1693
    Illegal SpeI site found at 260
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 260
    Illegal NotI site found at 995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 978
    Illegal XhoI site found at 248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1693
    Illegal SpeI site found at 260
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1693
    Illegal SpeI site found at 260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 903


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Categories
Parameters
None